Demonstrators blocked the entrance to the Jewish community of Ofra, north of Jerusalem, and also a segment of Highway 60 near the community in both directions on Wednesday night.
Reportedly the demonstrators were Jewish, and their reasons for the protest are unknown.
They dragged dozens of tires on to the road, and reportedly also added cinder blocks and boulders.
Israel Police spent some time dispersing the crowd and opening the road. Police then remained on site to prevent further disturbances as well.
Two of the teens were arrested on suspicion of organizing the road block and encouraging the rioters.
Highway 60 is a main artery which runs from north to south along the eastern part of the country, traveling through Samaria, Jerusalem and down through Judea to Be’er Sheva in southern Israel.
The highway is a favored venue of countless terrorists who target residents and travelers along the route in both directions from north to south.
In an attack on Wednesday night, Arab terrorists targeted an Israeli driver and passengers on a bus traveling on Highway 40.
No one was physically injured and no damage was reported.