Photo Credit: Courtesy of Hebrew University of Jerusalem
Professor Alexander (Sasha) Vainstein at the Hebrew University of Jerusalem’s Robert H. Smith Faculty of Agriculture, Food and Environment.

Around the world, the cannabis plant is gaining in popularity and legitimacy as a medical treatment for a broad range of illnesses. Now, researchers at the laboratory of Professor Alexander (Sasha) Vainstein at the Hebrew University of Jerusalem’s Robert H. Smith Faculty of Agriculture, Food and Environment, in partnership with and funding from Mariana Bioscience Ltd, have successfully engineered a cannabis plant with higher levels of medically-important substances, such as THC.

Tetrahydrocannabinol (THC) is the principal psychoactive constituent of cannabis. The status of THC as an illegal drug in most countries imposes restrictions on research material supply and funding, such as in the United States where the National Institute on Drug Abuse and Drug Enforcement Administration continue to control the sole federally-legal source of cannabis for researchers. Despite an August 2016 announcement that licenses would be provided to growers for supplies of medical marijuana, no such licenses were ever issued, despite dozens of applications.


Over in the Eternal City, the researchers successfully increased the level of THC by close to 17%, and the level of CBG (cannabigerol), often referred to as the mother of all cannabinoids, by close to 25%. Further, Vainstein and his team were able to increase the ratio of terpenes, which are responsible for maximizing the euphoric effects of cannabis, by 20–30%.

The stated goal of their study was to find a way to intervene in the biochemical pathways in the cannabis plant to increase or decrease the production of active substances. The researchers accomplished this by manipulating a plant-based virus that had first been neutralized so that it could not harm the plant and then manipulating it to express the genes that influence the production of active substances in the cannabis plant.

“This represents an innovative use of these tools, which were constructed using synthetic biology tools,” explained Vainstein. “Next, we developed an innovative technology based on infection with an engineered virus to facilitate chemical reactions that increase the quantities of desired substances. In collaboration with Mariana Bioscience Ltd., we examined the infected plants and found that the levels of the substances in question had indeed risen.”

This is the first time that researchers have succeeded in performing such a feat with cannabis plants.

Currently, there is a great deal of research activity aimed at identifying additional substances and medical treatments that can be derived from the cannabis plant, in addition to the more than 200 active ingredients that have already been identified. Until now, there was no way to tailor strains to produce certain cannabis substances or to alter the ratio between them.

According to Vainstein, “These study results will be valuable both to industry—to increase the yield of active substances, and to medical researchers—to cultivate and develop new strains for medical cannabis users.”

Vainstein added that more extensive experiments with the engineered plant are currently underway and should be available to cannabis industry leaders and medical researchers in the next few months.


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